Fast, cheap, accurate: Researchers pin hopes on Nobel Prize-winning CRISPR technology to detect coronavirus – USA TODAY

Posted: October 28, 2020 at 9:56 am

Scientists Emmanuelle Charpentier and Jennifer Doudna have won the Nobel Prize in chemistry for their pioneering work on the gene-editing tool CRISPR. The tool has been used to engineer better crops and to try to cure human diseases. (Oct. 7) AP Domestic

Coronavirus tests performed in labsare the gold standard for accuracy, and antigen tests are a fast and inexpensive alternative.

But backers of a third type of test, developed by a Nobel Prize winner usingcutting-edge CRISPR technology, say it has the potential to be all three:rapid, accurate and inexpensive.

Although these gene-editing technology testsare still being developed and won't be readyin the United States this yearas the weather cools and demandsurges, research groups recently published papers describing them as anappealing alternative as testing shortages persist amid the COVID-19 pandemic.

Dr. Jennifer Doudna, a University of California-Berkeley researcher whose pioneering work in CRISPR earned a share of this year's Nobel Prize in chemistry, said the test can be done quickly and doesn't require a lab.

"We have a ways to go before CRISPR-based diagnostics reach widespread use, but I believe well see an impact during the current pandemic," Doudna said. "Because it is simple to adjust these tests to detect other targets, the platform were developing now is laying the groundwork to deploy CRISPR for rapid diagnosis during future outbreaks."

Dr. Jennifer Doudna of the University of California-Berkeley earned a share of this year's Nobel Prize in chemistry for her pioneering work in CRISPR technology.(Photo: Susan Walsh, AP)

CRISPR, or clustered regularly interspaced short palindromic repeats, is a gene-editing technology studiedfor a wide range of uses from cancer and sickle cell disease treatments to improved food production.

In 2016, Doudna's lab developed a way to detect RNA using the technology.Her lab collaborated with Dr. Melanie Ott of San Francisco-based Gladstone Institutes to develop an HIV test, but when the pandemic hit, the researchers focused on developing a coronavirus test.

The test recognizesa sequence of RNA inSARS-CoV-2, the coronavirus that causes COVID-19.

Inan Oct. 12 publication, researchers reported the test yielded results infive minutes and correctly identified five samples from patients with the coronavirus. When used witha mobile phone to detect signals generated by thetest, the technology couldprovide a fast, low-cost test outside a laboratory, researchers saidin the paper, which was not peer-reviewed.

Massachusetts Institute of Technology scientists also are honing a CRISPR-based test that can be used outside a lab. In a New England Journal of Medicine letter published last month, researchers said the test was evaluated at a University of Washington lab using 202 samples with the coronavirus and 200 without. The test correctly identified 93.1% of positive samples. The test also had 98.5% specificity, which means it rarely reportedfalse positives.

Feluda, a paper-based CRISPRtest named after a fictional India detective, has been cleared by that nation'sdrug regulators for commercial launch. But it's unclear how the Indian conglomerate Tata Group plans to deploy the test in India, which trails only the United States with nearly 7.7 million cases, according to data from Johns Hopkins University.

GigiGronvall, a senior scholar at the Johns Hopkins Center for Health Security, said she expects more labs will explore tests using this technology.She called it"extremely promising" because people can use thetests outside the lab.

"That has been a big challenge with testing generally,"Gronvall said."People need to have their results pretty quickly otherwise they keep going about their day and they might be infectious and not stop having contact with other people."

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The south San Francisco-based biotechnology firm Mammoth Biosciences, co-founded by Doudna, is working to further develop its test and make it available to labs and medical providers. The companyreceived a National Institutes of Health grant to accelerate development.

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Mammoth CEO Trevor Martin said the company's goal is to make a test that is fast and accurate.

"Right now you have to make this choice: Do you want something that is simple and fast or something that has the highest accuracy?" Martin said.

The most accurate lab tests are molecular-basedpolymerase chain reaction, or PCR, which amplify a small amount of genetic material from anasal swab sample. Labs are limited in the number of PCR tests they can run because of periodic shortages of chemical reagents and other testing materials. In July, when demand outpaced labs' ability to perform tests, consumers whose tests were routed to major labs routinely waited a week or longer for results.

Antigen tests, which detect proteins of the coronavirus,can produce results in 15 minutes but are considered less sensitive than lab tests.

Martin saidCRISPR-based tests offer "a technology that is very simple, very fast yet extremely accurate."

Beyond the technology, test developers are trying to solve another challenge: designinga test that is easy to use and inexpensive.

Dr. Feng Zhang, an MIT biochemist and core member of the Broad Institutewhose lab is developing the StopCOVID test, said his team wants to make a low-cost device that can work with a disposable cartridge. Collaborators wanta testeasy enough for consumers to use at home by takingtheir own nasal swab.

"The challenge is to work out how to scale up the manufacturing so that we can get the cost to be as low as possible," Zhang said.

He said his collaborators are working to bring the test to market as soon as possible. A version of the test already is being used by a Thailand hospital to screen patients.

Mammoth's Martin said designing a test for wide useis "where a lot of things stumble."

"Its really nice to have a new technology but you need to get it to the places where its needed," he said. "Thats something weve been working on very diligently."

Dr. Sophia L. Yohe,director of the University of Minnesotas Molecular Diagnostics Laboratory, said it'sunknown whether test developers will be able to streamline steps to allow a large number of tests.

Some early versions of CRISPR tests required samples to be extracted and amplified, similar to a lab-based PCR tests, saidYohe, chair of the College of American Pathologists Personalized Health Care Committee.

"If you can do only one test at a time, even if it only takes 30 minutes, suddenly doing a thousand tests takes a long time," she said. "So scalability I think is an issue that has to be considered if you are looking at doing high volumes of testing."

Ken Alltucker is on Twitter as@kalltuckeror can be emailed at alltuck@usatoday.com

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