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CAR T-Cell Immunotherapy for ALL – National Cancer Institute

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For years, the cornerstones of cancer treatment have been surgery, chemotherapy, and radiation therapy. Over the last decade, targeted therapies like imatinib (Gleevec) and trastuzumab (Herceptin)drugs that target cancer cells by homing in on specific molecular changes seen primarily in those cellshave also emerged as standard treatments for a number of cancers.

Illustration of the components of second- and third-generation chimeric antigen receptor T cells. (Adapted by permission from the American Association for Cancer Research: Lee, DW et al. The Future Is Now: Chimeric Antigen Receptors as New Targeted Therapies for Childhood Cancer. Clin Cancer Res; 2012;18(10); 278090. doi:10.1158/1078-0432.CCR-11-1920)

And now, despite years of starts and stutter steps, excitement is growing for immunotherapytherapies that harness the power of a patients immune system to combat their disease, or what some in the research community are calling the fifth pillar of cancer treatment.

One approach to immunotherapy involves engineering patients own immune cells to recognize and attack their tumors. And although this approach, called adoptive cell transfer (ACT), has been restricted to small clinical trials so far, treatments using these engineered immune cells have generated some remarkable responses in patients with advanced cancer.

For example, in several early-stage trials testing ACT in patients with advanced acute lymphoblastic leukemia (ALL) who had few if any remaining treatment options, many patients cancers have disappeared entirely. Several of these patients have remained cancer free for extended periods.

Equally promising results have been reported in several small trials involving patients with lymphoma.

These are small clinical trials, their lead investigators cautioned, and much more research is needed.

But the results from the trials performed thus far are proof of principle that we can successfully alter patients T cells so that they attack their cancer cells, said one of the trial's leaders, Renier J. Brentjens, M.D., Ph.D., of Memorial Sloan Kettering Cancer Center (MSKCC) in New York.

Adoptive cell transfer is like giving patients a living drug, continued Dr. Brentjens.

Thats because ACTs building blocks are T cells, a type of immune cell collected from the patients own blood. After collection, the T cells are genetically engineered to produce special receptors on their surface called chimeric antigen receptors (CARs). CARs are proteins that allow the T cells to recognize a specific protein (antigen) on tumor cells. These engineered CAR T cells are then grown in the laboratory until they number in the billions.

The expanded population of CAR T cells is then infused into the patient. After the infusion, if all goes as planned, the T cells multiply in the patients body and, with guidance from their engineered receptor, recognize and kill cancer cells that harbor the antigen on their surfaces.

Although adoptive cell transfer has been restricted to small clinical trials so far, treatments using these engineered immune cells have generated some remarkable responses in patients with advanced cancer.

This process builds on a similar form of ACT pioneered by Steven Rosenberg, M.D., Ph.D., and his colleagues from NCIs Surgery Branch for patients with advanced melanoma.

The CAR T cells are much more potent than anything we can achieve with other immune-based treatments being studied, said Crystal Mackall, M.D., of NCIs Pediatric Oncology Branch (POB).

Even so, investigators working in this field caution that there is still much to learn about CAR T-cell therapy. But the early results from trials like these have generated considerable optimism.

CAR T-cell therapy eventually may become a standard therapy for some B-cell malignancies like ALL and chronic lymphocytic leukemia, Dr. Rosenberg wrote in a Nature Reviews Clinical Oncology article.

More than 80 percent of children who are diagnosed with ALL that arises in B cellsthe predominant type of pediatric ALLwill be cured by intensive chemotherapy.

For patients whose cancers return after intensive chemotherapy or a stem cell transplant, the remaining treatment options are close to none, said Stephan Grupp, M.D., Ph.D., of the Childrens Hospital of Philadelphia (CHOP) and the lead investigator of a trial testing CAR T cells primarily in children with ALL. This treatment may represent a much-needed new option for such patients, he said.

Trials of CAR T cells in adults and children with leukemia and lymphoma have used T cells engineered to target the CD19 antigen, which is present on the surface of nearly all B cells, both normal and cancerous.

In the CHOP trial, which is being conducted in collaboration with researchers from the University of Pennsylvania, all signs of cancer disappeared (a complete response) in 27 of the 30 patients treated in the study, according to findings published October 16 in the New England Journal of Medicine.

Nineteen of the 27 patients with complete responses have remained in remission, the study authors reported, with 15 of these patients receiving no further therapy and 4 patients withdrawing from the trial to receive other therapy.

According to the most recent data from a POB trial that included children with ALL, 14 of 20 patients had a complete response. And of the 12 patients who had no evidence of leukemic cells, called blasts, in their bone marrow after CAR T-cell treatment, 10 have gone on to receive a stem cell transplant and remain cancer free, reported the studys lead investigator, Daniel W. Lee, M.D., also of the POB.

Dr. Crystal Mackall

Our findings strongly suggest that CAR T-cell therapy is a useful bridge to bone marrow transplant for patients who are no longer responding to chemotherapy, Dr. Lee said.

Similar results have been seen in phase I trials of adult patients conducted at MSKCC and NCI.

In findings published in February 2014, 14 of the 16 participants in the MSKCC trial treated to that point had experienced complete responses, which in some cases occurred 2 weeks or sooner after treatment began. Of those patients who were eligible, 7 underwent a stem cell transplant and are still cancer free.

The NCI-led trial of CAR T cells included 15 adult patients, the majority of whom had advanced diffuse large B-cell lymphoma. Most patients in the trial had either complete or partial responses, reported James Kochenderfer, M.D., and his NCI colleagues.

Our data provide the first true glimpse of the potential of this approach in patients with aggressive lymphomas that, until this point, were virtually untreatable, Dr. Kochenderfer said. [NCI Surgery Branch researchers have also reported promising results from one of the first trials testing CAR T cells derived from donors, rather than the patients themselves, to treat leukemia and lymphoma.]

Other findings from the trials have been encouraging, as well. For example, the number of CAR T cells increased dramatically after infusion into patients, as much as 1,000-fold in some individuals. In addition, after infusion, CAR T cells were detected in the central nervous system, a so-called sanctuary site where solitary cancer cells that have evaded chemotherapy or radiation may hide. In two patients in the NCI pediatric trial, the CAR T-cell treatment eradicated cancer that had spread to the central nervous system.

If CAR T cells can persist at these sites, it could help fend off relapses, Dr. Mackall noted.

CAR T-cell therapy can cause several worrisome side effects, perhaps the most troublesome being cytokine-release syndrome.

The infused T cells release cytokines, which are chemical messengers that help the T cells carry out their duties. With cytokine-release syndrome, there is a rapid and massive release of cytokines into the bloodstream, which can lead to dangerously high fevers and precipitous drops in blood pressure.

Cytokine-release syndrome is a common problem in patients treated with CAR T cells. In the POB and CHOP trials, patients with the most extensive disease prior to receiving the CAR T cells were more likely to experience severe cases of cytokine-release syndrome.

For most patients, trial investigators have reported, the side effects are mild enough that they can be managed with standard supportive therapies, including steroids.

The research team at CHOP noticed that patients experiencing severe reactions all had particularly high levels of IL-6, a cytokine that is secreted by T cells and macrophages in response to inflammation. So they turned to two drugs that are approved to treat inflammatory conditions like juvenile arthritis: etanercept (Enbrel) and tocilizumab (Actemra), the latter of which blocks IL-6 activity.

The patients had excellent responses to the treatment, Dr. Grupp said. We believe that [these drugs] will be a major part of toxicity management for these patients.

The other two teams subsequently used tocilizumab in several patients. Dr. Brentjens agreed that both drugs could become a useful way to help manage cytokine-release syndrome because, unlike steroids, they dont appear to affect the infused CAR T cells activity or proliferation.

Even with these encouraging preliminary findings, more research is needed before CAR T-cell therapy becomes a routine option for patients with ALL.

We need to treat more patients and have longer follow-up to really say what the impact of this therapy is [and] to understand its true performance characteristics, Dr. Grupp said.

We need to treat more patients and have longer follow-up to really say what the impact of this therapy is [and] to understand its true performance characteristics.

Dr. Stephan Grupp

Several other trials testing CAR T cells in children and adults are ongoing and, with greater interest and involvement from the pharmaceutical and biotechnology sector, more trials testing CAR T cells are being planned.

Researchers are also studying ways to improve on the positive results obtained to date, including refining the process by which the CAR T cells are produced.

Research groups like Dr. Brentjens are also working to make a superior CAR T cell, including developing a better receptor and identifying better targets.

For example, Dr. Lee and his colleagues at NCI have developed CAR T cells that target the CD22 antigen, which is also present on most B cells, although in smaller quantities than CD19. The CD22-targeted T cells, he believes, could be used in concert with CD19-targeted T cells as a one-two punch in ALL and other B-cell cancers. NCI researchers hope to begin the first clinical trial testing the CD22-targeted CAR T cells in November 2014.

Based on the success thus far, several research groups across the country are turning their attention to developing engineered T cells for other cancers, including solid tumorslike pancreatic and brain cancers.

The stage has now been set for greater progress, Dr. Lee believes.

NCI investigators, for example, now have a platform to plug and play better CARs into that system, without a lot of additional R&D time, he continued. Everything else should now come more rapidly.

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CAR T-Cell Immunotherapy for ALL - National Cancer Institute

Gene Therapy and Cell Therapy Defined | ASGCT – American …

Gene therapy and cell therapy are overlapping fields of biomedical research with the goals of repairing the direct cause of genetic diseases in the DNA or cellular population, respectively. These powerful strategies are also being focused on modulating specific genes and cell subpopulations in acquired diseases in order to reestablish the normal equilibrium. In many diseases, gene and cell therapy are combined in the development of promising therapies.

In addition, these two fields have helped provide reagents, concepts, and techniques that are elucidating the finer points of gene regulation, stem cell lineage, cell-cell interactions, feedback loops, amplification loops, regenerative capacity, and remodeling.

Gene therapy is defined as a set of strategies that modify the expression of an individuals genes or that correct abnormal genes. Each strategy involves the administration of a specific DNA (or RNA).

Cell therapy is defined as the administration of live whole cells or maturation of a specific cell population in a patient for the treatment of a disease.

Gene therapy: Historically, the discovery of recombinant DNA technology in the 1970s provided the tools to efficiently develop gene therapy. Scientists used these techniques to readily manipulate viral genomes, isolate genes, identify mutations involved in human diseases, characterize and regulate gene expression, and engineer various viral vectors and non-viral vectors. Many vectors, regulatory elements, and means of transfer into animals have been tried. Taken together, the data show that each vector and set of regulatory elements provides specific expression levels and duration of expression. They exhibit an inherent tendency to bind and enter specific types of cells as well as spread into adjacent cells. The effect of the vectors and regulatory elements are able to be reproduced on adjacent genes. The effect also has a predictable survival length in the host. Although the route of administration modulates the immune response to the vector, each vector has a relatively inherent ability, whether low, medium or high, to induce an immune response to the transduced cells and the new gene products.

The development of suitable gene therapy treatments for many genetic diseases and some acquired diseases has encountered many challenges and uncovered new insights into gene interactions and regulation. Further development often involves uncovering basic scientific knowledge of the affected tissues, cells, and genes, as well as redesigning vectors, formulations, and regulatory cassettes for the genes.

While effective long-term treatments for anemias, hemophilia, cystic fibrosis, muscular dystrophy, Gauschers disease, lysosomal storage diseases, cardiovascular diseases, diabetes, and diseases of the bones and joints are elusive today, some success is being observed in the treatment of several types of immunodeficiency diseases, cancer, and eye disorders. Further details on the status of development of gene therapy for specific diseases are summarized here.

Cell therapy: Historically, blood transfusions were the first type of cell therapy and are now considered routine. Bone marrow transplantation has also become a well-established protocol. Bone marrow transplantation is the treatment of choice for many kinds of blood disorders, including anemias, leukemias, lymphomas, and rare immunodeficiency diseases. The key to successful bone marrow transplantation is the identification of a good "immunologically matched" donor, who is usually a close relative, such as a sibling. After finding a good match between the donors and recipients cells, the bone marrow cells of the patient (recipient) are destroyed by chemotherapy or radiation to provide room in the bone marrow for the new cells to reside. After the bone marrow cells from the matched donor are infused, the self-renewing stem cells find their way to the bone marrow and begin to replicate. They also begin to produce cells that mature into the various types of blood cells. Normal numbers of donor-derived blood cells usually appear in the circulation of the patient within a few weeks. Unfortunately, not all patients have a good immunological matched donor. Furthermore, bone marrow grafts may fail to fully repopulate the bone marrow in as many as one third of patients, and the destruction of the host bone marrow can be lethal, particularly in very ill patients. These requirements and risks restrict the utility of bone marrow transplantation to some patients.

Cell therapy is expanding its repertoire of cell types for administration. Cell therapy treatment strategies include isolation and transfer of specific stem cell populations, administration of effector cells, induction of mature cells to become pluripotent cells, and reprogramming of mature cells. Administration of large numbers of effector cells has benefited cancer patients, transplant patients with unresolved infections, and patients with chemically destroyed stem cells in the eye. For example, a few transplant patients cant resolve adenovirus and cytomegalovirus infections. A recent phase I trial administered a large number of T cells that could kill virally-infected cells to these patients. Many of these patients resolved their infections and retained immunity against these viruses. As a second example, chemical exposure can damage or cause atrophy of the limbal epithelial stem cells of the eye. Their death causes pain, light sensitivity, and cloudy vision. Transplantation of limbal epithelial stem cells for treatment of this deficiency is the first cell therapy for ocular diseases in clinical practice.

Several diseases benefit most from treatments that combine the technologies of gene and cell therapy. For example, some patients have a severe combined immunodeficiency disease (SCID) but unfortunately, do not have a suitable donor of bone marrow. Scientists have identified that patients with SCID are deficient in adenosine deaminase gene (ADA-SCID), or the common gamma chain located on the X chromosome (X-linked SCID). Several dozen patients have been treated with a combined gene and cell therapy approach. Each individuals hematopoietic stem cells were treated with a viral vector that expressed a copy of the relevant normal gene. After selection and expansion, these corrected stem cells were returned to the patients. Many patients improved and required less exogenous enzymes. However, some serious adverse events did occur and their incidence is prompting development of theoretically safer vectors and protocols. The combined approach also is pursued in several cancer therapies.

Further information on the progress and status of gene therapy and cell therapy on various diseases is listed here.

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Gene Therapy and Cell Therapy Defined | ASGCT - American ...

StemCell Therapy MD

Colorado Clinic offers multiple regenerative medicine stem cell treatments. These treatments are provided as an outpatient by a Double Board Certified Doctor. Each treatment maintains minimal risk, with the possibility of providing repair and healing of injured tendons, ligaments, cartilage and muscle.

Click on the Treatments on the Left Tabs for more information.

Stem Cell Treatments at Colorado Clinic

Traditional therapies for osteoarthritis, ligament injury and tendonitis maintain certain commonalities. They help provide excellent pain relief, however, they do not alter the condition or help with the healing process. They act as an excellent band aid, but they do not REPAIR the problem!

The newest treatments for helping repair the damage involve Regenerative Medicine. The therapies are cutting edge and include stem cells, platelets, growth factors and cytokines.

Here is an example of what regenerative medicine offers. When a football player sustains a rotator cuff tendon injury, it may heal by itself in six to 10 weeks. Healing of damaged tendons or ligaments may occur naturally. However, it does not reach 100% strength like it was before.

With regenerative medicine, this situation may be very different. Healing of the rotator cuff injury may occur much faster, and it may reach 100% normal strength. This can help prevent future injury and get patients back on the field faster.

Regenerative treatments may permit patients to avoid or delay the need for surgery when it comes to all sorts of injury. The most common of these is degenerative arthritis. Joint replacement surgery is not without risk, therefore, stem cell treatments may help repair some of the cartilage damage while providing substantial pain relief.

With minimal risk, outpatient stem cell treatments offer a substantial upside. Make your appointment at Colorado Clinic today!

Amniotic Stem Cell Injections

Life comes from birth. Its one of the most commonly accepted rules in our society. But can the birth process offer even more? As research and science evolved over time, studies have shown that amniotic stem cells can have a revolutionary effect on the human recovery process.

First, lets look at what amniotic stem cells are. Stem cells are the basic components (cells) of our human body. One of their most amazing characteristic is that they can become almost any type of cell, from muscle to bone or skin cell.

Amniotic stem cells are obtained from the amniotic fluid, which is produced during a caesarean birth. During pregnancy, the amniotic fluid protects the fetus and it feeds it with the necessary supplements needed to sustain life and development. A while back, this fluid was normally discarded, but once researchers got to understand its amazing therapeutic benefits, now its collected and stored because of its high concentration of pluripotent stem cells.

Amniotic derived stem cell fluid comes from consenting donors and is processed at an FDA regulated lab. It is checked for all sorts of diseases prior to being accepted for use in others.

Although stem cells have been used for decades, regenerative therapy is fairly new, and sometimes pushes the boundaries of human imagination and perception. Following the use of amniotic stem cell injections, more evidence reveals exciting results in muscle repair and pain relief which has made amniotic stem cells possibly the holy grail in treatment.

Amniotic stem cell injections offer the ability to heal damaged tissue naturally. The tissue regeneration and repair properties of the amniotic stem cell fluid are an effective anti-inflamatory that relieves pain and contains natural growth factors that assist in healthy tissue growth. Moreover, the hyaluronic acid that is also in amniotic fluid is an important component of the joint fluid that helps cartilage growth. Amniotic fluid is also a great source of stem cells, found in a much higher concentration than the adult bone marrow. And just like when one uses their own stem cells, the use of amniotic fluid doesnt cause rejection or allergic reaction when injected into a patient.

Amniotic stem cell injections have been getting more attention since they have been openly used by prominent athletes with impressive results and even a few saved careers! The ability to safely and effectively treat painful and debilitating injuries and conditions of the knees, elbows, and shoulders without lengthy rehabilitation or recovery time isnt just appealing to professional athletes, but to anyone who wants relief from pain and to return to their favorite activities.

Initial small studies are showing that amniotic stem cell injections work well for the following indications: 1) Tendonitis 2) Ligament Injury 3) Arthritis 4) Sports Medicine Injuries 5) Cartilage Defects

Dr. Sisson at Colorado Clinic is an expert in regenerative medicine treatments. Call the practice today for an appointment!

Bone Marrow Derived Stem Cell Injections for Musculoskeletal Problems

What are Bone Marrow Derived Stem Cell Injections?

There are many types of stem cell injections that are currently in research mode. One type of stem cell injection currently used for many types of degenerative conditions is the bone marrow derived stem cell injection. This type of stem cell treatment is excellent for degenerative disc disease, joint arthritis, ligament injuries, spinal arthritis, and tendonitis. Studies have shown that therapy using regenerative treatment, such as bone marrow stem cell injections, work well for degenerative conditions.

Bone Marrow Derived Stem Cell Collection and Injection

Bone marrow derived stem cell injections are an outpatient procedure where a patients bone marrow is harvested. It is then processed and injected into the area of concern in the same setting. In bone marrow derived stem cell injections, collection is done in an outpatient procedure which takes about 30 minutes. The bone marrow derived stem cells are collected using a catheter and local anesthetic.

The bone marrow derived stem cells are removed from the body in the blood, circulated through a machine with the filtered blood, and returned to the patients body in the same procedure. The stem cells are filtered out of the blood using the aspheresis machine, which retains only the stem cells.

What is the Future of Bone Marrow Derived Stem Cell Injections?

The future of bone marrow derived stem cell injections is a bright one. There are two types of bone marrow stem cells that can be derived from the tissue composing the middle of the bones, mesenchymal, and hematopoietic stem cells. It is the hematopoietic stem cells that differentiate back into blood cells among other things, and the mesenchymal cells that differentiate into skeletal and vertebral tissues.

Bone marrow derived stem cell injections are showing excellent results for tendonitis, ligament injuries and degenerative arthritis. This can help produce great results for athletes and individuals who desire to avoid or delay the need for joint replacement surgery.

Dr. Sisson at Colorado Clinic is at the forefront of regenerative medicine treatments with stem cells. You will be in good hands!

PRP Therapy at Colorado Clinic

The Facts about PRP Injections

Platelet-rich (PRP) therapy is a form of therapy that is used for damage that occurs within the tendons, ligaments, and joints. This type of therapy works by stimulating repair within the areas that are damaged, while also providing pain relief for the area where the therapy is used. PRP therapy has been around for quite some time, but has only recently become a more common method of treatment for musculoskeletal conditions.

Due to the ease of application, and the very few side-effects present with PRP therapy, it is commonly replacing other treatments that are more invasive, such as surgical procedures.

What exactly is PRP Therapy?

PRP therapy is often called platelet-rich plasma therapy, and this type of therapy is provided in the form of an injection. Initially, about 30-60cc of blood is drawn from the patients arm. It is placed into a centrifuge machine and separates into several layers. The middle layer contains concentrated platelets and growth factors and is used in the treatment for injection into the problem area.

Your blood is composed of several different parts, and when the blood is put into a medical machine that spins it at a fast rate, the platelets are separated from the blood, collected, and then put into a vial in a concentrated amount. The collected platelets are then injected into the area that is damaged, which provides the pain relief and repairing effects for the area. This allows the patient to get the platelets and growth factors needed for healing, while also using the bodys own resources, which eliminates the possibility of side-effects occurring due to the body rejecting the injection that is made.

The platelets that are removed from the blood are the same ones within the blood that stick to one another when we are injured and the blood clots. While the blood as a whole is known to have great healing powers, the platelets are one of the most effective healing components of the blood. When injected into the different damaged areas of the body, they are able to call in stem cells, and also allow for regeneration of the soft tissue.

How does PRP Therapy Work?

When the PRP injection is made, the solution goes directly into the area that is damaged, and also into the areas surrounding the damage. The therapy is known to provide pain relief within a week for patients in up to 80% of cases, due to the ability ofthe injections to stimulate healing in the area at a much faster rate than what your body is able to provide. Platelet rich plasma also contains significant amounts of growth factors, and even severe damage can be healed over time with the use of this form of therapy.

Where can PRP be Used?

PRP therapy can be used in all of the joints within the body, and even areas of soft tissue that are damaged such as the shoulder, elbow, achilles, etc. This may include tendonitis, ligament injury or degenerative arthritis.

Platelet-rich plasma therapy at Colorado Clinic is offered by the top pain management and regenerative medicine doctor in Northern Colorado, Dr. Sisson. He has extensive experience with regenerative medicine including PRP therapy, make your appointment today!

Continued here: Regenerative Medicine Colorado Clinic

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StemCell Therapy MD

Stem Cell Therapy in Mexico

Stem Cell MX is dedicated to providing COPD and heart disease patients with information about stem cell therapy at Angeles Health International, Mexicos largest private hospital network.

Stem Cell Therapy is a fast growing area of medical research. Research into how stem cells can cure a number of conditions has been extensive over the past 3 decades and here at Stem Cell MX we are proud to be at the forefront of breakthrough discoveries and treatments. We dedicate ourselves to providing you with information about Stem Cells and what they can do for you.

At Stem Cell MX we can use Stem Cell therapy to treat 11 core treatable conditions including chronic obstructive pulmonary disease (COPD), heart conditions and joint conditions, such as osteoarthritis. We use two types of stem cell programs; autologous, meaning that we use your own stem cells, and allogeneic, where we use donated adult stem cells from one of the best labs in the world.

Stem cell research has had bad press over the years due to the misconception that Stem Cells can only come from embryos. This isnt true. Here at Stem Cell MX we only use Adult Stem Cells which have been harvested from either the donor or the patients themselves.

If you want to find out more about stem cell therapy with no obligation then contact us today. Our stem cell clinical trials are based on thirty years of research and clinical experience conducted by leading researchers and clinicians in Europe and the United States.

To find out the basics about stem cells read An Introduction to Stem Cells

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Stem Cell Therapy in Mexico

Autologous Adipose Tissue Derived Stromal Vascular Fraction Cells Application In Patients – Video

Autologous Adipose Tissue Derived Stromal Vascular Fraction Cells Application In Patients
The U.S. Stem Cell Clinic is founded on the principle belief that the quality of life for our patients can be improved through stem cell therapy. We are dedicated to providing safe and effective...

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Autologous Adipose Tissue Derived Stromal Vascular Fraction Cells Application In Patients - Video

Platelet Rich Plasma Injections For Chronic Pain Relief May Help You Avoid Sugery – Video

Platelet Rich Plasma Injections For Chronic Pain Relief May Help You Avoid Sugery (541) 716-6469 Dr David Russo with Columbia Pain Management talks about the use of platelet rich plasma injections for chronic pain relief. Stem Cell Therapy can...

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Platelet Rich Plasma Injections For Chronic Pain Relief May Help You Avoid Sugery - Video

Stem Cell Treatment for COPD | StemRx Bioscience Solutions – Video

Stem Cell Treatment for COPD | StemRx Bioscience Solutions
Dr.P V Mahajan expertise in Stem Cell Therapy | For More details please visit

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Stem Cell Treatment for COPD | StemRx Bioscience Solutions - Video

Stem Cell Therapy Market in Asia-Pacific to 2018 – Video

Stem Cell Therapy Market in Asia-Pacific to 2018
GBI Research, the leading business intelligence provider, has released its latest research Stem Cell Therapy Market in Asia-Pacific to 2018 - Commercialization Supported by Favorable Government...

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Stem Cell Therapy Market in Asia-Pacific to 2018 - Video

STEM CELL therapy incredible results for severe MS – Video

STEM CELL therapy incredible results for severe MS
get some STEM CELL on ya! some basics on the buzz!

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STEM CELL therapy incredible results for severe MS - Video

Phytoscience Philipine celebrity Share good effect of stem cell Therapy – Video

Phytoscience Philipine celebrity Share good effect of stem cell Therapy
for more infor about the products visit reach us: 0927-2329074 / 0923-6062834 / (02) 463-9400 like us:

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Clinical Benefits of Stem Cell Therapy – Video

Clinical Benefits of Stem Cell Therapy
Stem cell therapy has made medical breakthroughs. Watch to see the clinical benefits of stem cell therapy.

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Clinical Benefits of Stem Cell Therapy - Video

NYC Health & Longevity Center Now Offering Stem Cell Therapy to Avoid Joint Replacement

NYC, NY (PRWEB) April 13, 2015

NYC Health & Longevity Center is now offering outpatient stem cell therapy to help patients avoid joint replacement in all extremities. The treatments are performed by a Board Certified physician, with most patients being able to avoid or delay the need for surgery. Simply call (844) GET-STEM for more information and scheduling with stem cell therapy NYC trusts.

Millions of joint replacements are performed in the US annually for degenerative arthritis of the knee, hip, shoulder, elbow, wrist and ankle. While these are mostly effective, they are not risk free procedures and should be avoided as long as possible. In addition, the implants placed are not meant to last forever.

With stem cell therapy now being commercially available, individuals now have access to the most cutting-edge procedures with the potentially to actually regenerate damaged tissue. This includes cartilage, ligament and tendon.

The stem cell procedures are performed by a Board Certified Anti-Aging doctor with considerable experience in both the stem cell procedures along with prolotherapy too.

The stem cell material comes from amniotic fluid that is obtained from consenting donors after a scheduled C-section, which is then processed at an FDA regulated lab. No fetal tissue or embryonic stem cells are used, eliminating any ethical concerns. Amniotic fluid causes no rejection, and has a very high amount of stem cells, growth factors and anti-inflammatory effects. The overall result is typically tremendous pain reduction and functional improvements that are long lasting.

Stem cell therapy for arthritis is performed on an outpatient basis, with absolutely minimal risk. The procedure takes less than a half hour, with patients able to return to desired activities quickly.

Along with degenerative arthritis, the stem cell procedures also help rheumatoid arthritis along with tendonitis of the rotator cuff, Achilles, elbow and knee. Athletes benefit from typically being able to avoid surgery and get back their sport much faster than with conventional treatments.

For more information on stem cell therapy at NYC Health & Longevity Center for extremity arthritis of the hips, knees, shoulders, elbow, wrist or ankle, call (844) GET-STEM.

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NYC Health & Longevity Center Now Offering Stem Cell Therapy to Avoid Joint Replacement

U.S. Stem Cell Clinic: What Conditions Can Be Treated? – Video

U.S. Stem Cell Clinic: What Conditions Can Be Treated?
tem cells have the unique attribute to form many different types of tissue including bone, cartilage, and muscle. They are naturally anti-inflammatory and can therefore help in the body #39;s...

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U.S. Stem Cell Clinic: What Conditions Can Be Treated? - Video

Stem Cell Therapy In USA

Who We Are at World Stem Cells, LLC

Advanced stem cell treatments offered by Would Stem Cells, LLC a patient management company for qualified patients at the medical facilities World Stem Cells Clinic, http://worldstemcellsclinic.comin Cancun provides an opportunity for a better quality of life. The clinic and laboratory were designed, built and are operated under the stringent guidelines as established by USFederal Regulations Title 21, Subpart C, 211-42 through 211-58,and the US Federal Drug Administrations Good Tissue Practice (cGTP)regulations for pharmaceutical, biologics and clinical laboratories. The strict adherence to these established guidelines and policies guarantees the highest quality of clinical care and stem cell treatment safety for you. Check out our clinic locations at

What Is Done

World Stem Cells Clinics medical staff and clinical physicians will examine you and review all available medical records, radiology films, CT scans and other diagnostic information to assess if stem cell therapy will be a helpful primary treatment or adjunctive therapy for your specific condition.

Then, the medical doctors meet and confer with the research scientists for a pre-treatment planning meeting. This Stem cell treatment planning conference takes advantage of decades of the staffs clinical experience, your current condition, your available social support system, full review of your medical history as well as an inclusion and consideration of any recently published research literature on stem cell treatments. In other words, you are provided a detailed, systematic and entirely unique treatment care plan for his or her needs.

Creating the best treatment

Sorry, they do not perform a one or two day treatment as it would not be medically sound and could not provide the benefits or safety that the World Stem Cells Clinic treatment schedule gives (please do not be fooled). Your Stem Cell Treatment at World Stem Cells Clinic takes 5 days to complete as the treatments are comprehensive and designed to maximize the benefits and safety you derive from the process.

How Is It Done

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Stem Cell Therapy In USA

Stem Cell Therapy for Pain – Now Available at Columbia Pain Management – Video

Stem Cell Therapy for Pain - Now Available at Columbia Pain Management Call: 541-716-6469 Columbia Pain Management Can Help You Get Your Life Back. Stem Cell Therapy for Pain - Now Available at Columbia Pain Management.

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Stem Cell Therapy for Pain - Now Available at Columbia Pain Management - Video

What is Stem Cell Therapy- Trinity Spine and Wellness Center New Port Richey – Video

What is Stem Cell Therapy- Trinity Spine and Wellness Center New Port Richey -727-372-9922 what is Stem Cell Therapy for back pain? Watch this video to see how Stem Cell Therapy can immediately help your chronic neck and back pain. Visit our website ...

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What is Stem Cell Therapy- Trinity Spine and Wellness Center New Port Richey - Video

U.S. Stem Cell Clinic: How long will my recovery take? – Video

U.S. Stem Cell Clinic: How long will my recovery take?
Our U.S. Stem Cell Clinic procedure is minimally invasive and patients walk out within 3 hours in most cases. As a result recovery time is very quick. Many patients will experience soreness...

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U.S. Stem Cell Clinic: How long will my recovery take? - Video

U.S. Stem Cell Clinic: Stem Cell Banking – Video

U.S. Stem Cell Clinic: Stem Cell Banking
The U.S. Stem Cell Clinic is founded on the principle belief that the quality of life for our patients can be improved through stem cell therapy. We are dedicated to providing safe and effective...

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U.S. Stem Cell Clinic: Stem Cell Banking - Video

U.S. Stem Cell Clinic: What is Stem Cell Therapy? – Video

U.S. Stem Cell Clinic: What is Stem Cell Therapy?
What is Stem Cell Therapy? Stem cell therapy attempts to harness the body #39;s own healing potential by isolating stem cells from one location of the body (fat tissue or bone marrow) and relocating...

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U.S. Stem Cell Clinic: What is Stem Cell Therapy? - Video

U.S. Stem Cell Clinic: How is Stem Cell Therapy Performed? – Video

U.S. Stem Cell Clinic: How is Stem Cell Therapy Performed?
Our U.S. Stem Cell Clinic will perform outpatient procedures using a process in which we isolate a patient #39;s own stem cells from either their own adipose fat tissue or bone marrow. Approximately...

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U.S. Stem Cell Clinic: How is Stem Cell Therapy Performed? - Video

U.S. Stem Cell Clinic: When should I expect to see results? – Video

U.S. Stem Cell Clinic: When should I expect to see results?
It is important to note that we are treating patients with their own adult stem cells, therefore each treatment and response is unique to that patient. No guarantee can be made of what results...

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U.S. Stem Cell Clinic: When should I expect to see results? - Video

Can PRP and Stem Cell Therapy Help You? | Orlando Orthopaedic Center – Video

Can PRP and Stem Cell Therapy Help You? | Orlando Orthopaedic Center
How can PRP and stem cell therapy help you heal? Orlando Orthopaedic Center #39;s Dr. Matthew R. Willey explains. For more visit

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Can PRP and Stem Cell Therapy Help You? | Orlando Orthopaedic Center - Video

Induced Pluripotent Stem Cell (iPSC) Industry Complete Report 2015 – 2016

DALLAS, April 2, 2015 /PRNewswire/ -- adds "Complete 2015-16 Induced Pluripotent Stem Cell (iPSC) Industry Report" in its store. Recent months have seen the first iPSC clinical trial in humans, creation of the world's largest iPSC Biobank, major funding awards, a historic challenge to the "Yamanaka Patent", a Supreme Court ruling affecting industry patent rights, the announcement of an iPSC cellular therapy clinic scheduled to open in 2019, and much more. Furthermore, iPSC patent dominance continues to cluster in specific geographic regions, while clinical trial and scientific publication trends give clear indicators of what may happen in the industry in 2015 and beyond.

Is it worth it to get informed about rapidly-evolving market conditions and identify key industry trends that will give an advantage over the competition?

BrowsetheReportComplete 2015-16 Induced Pluripotent Stem Cell (iPSC) Industry Reportat ....

Induced pluripotent stem cells represent a promising tool for use in the reversal and repair of many previously incurable diseases. The cell type represents one of the most promising advances discovered within the field of stem cell research during the past decade, making this a valuable industry report for both companies and investors to claim in order to optimally position themselves to sell iPSC products. To profit from this lucrative and rapidly expanding market, you need to understand your key strengths relative to the competition, intelligently position your products to fill gaps in the market place, and take advantage of crucial iPSC trends.

Report Applications

This global strategic report is produced for: Management of Stem Cell Product Companies, Management of Stem Cell Therapy Companies, Stem Cell Industry Investors

It is designed to increase your efficiency and effectiveness in:

Four Primary Areas of Commercialization

There are currently four major areas of commercialization for induced pluripotent stem cells, as described below:

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Induced Pluripotent Stem Cell (iPSC) Industry Complete Report 2015 - 2016

Stem Cell Therapy for Neuromuscular Diseases | InTechOpen

1. Introduction

Neuromuscular disease is a very broad term that encompasses many diseases and aliments that either directly, via intrinsic muscle pathology, or indirectly, via nerve pathology, impair the functioning of the muscles. Neuromuscular diseases affect the muscles and/or their nervous control and lead to problems with movement. Many are genetic; sometimes, an immune system disorder can cause them. As they have no cure, the aim of clinical treatment is to improve symptoms, increase mobility and lengthen life. Some of them affect the anterior horn cell, and are classified as acquired (e.g. poliomyelitis) and hereditary (e.g. spinal muscular atrophy) diseases. SMA is a genetic disease that attacks nerve cells, called motor neurons, in the spinal cord. As a consequence of the lost of the neurons, muscles weakness becomes to be evident, affecting walking, crawling, breathing, swallowing and head and neck control. Neuropathies affect the peripheral nerve and are divided into demyelinating (e.g. leucodystrophies) and axonal (e.g. porphyria) diseases. Charcot-Marie-Tooth (CMT) is the most frequent hereditary form among the neuropathies and its characterized by a wide range of symptoms so that CMT-1a is classified as demyelinating and CMT-2 as axonal (Marchesi & Pareyson, 2010). Defects in neuromuscular junctions cause infantile and non-infantile Botulism and Myasthenia Gravis (MG). MG is a antibody-mediated autoimmune disorder of the neuromuscular junction (NMJ) (Drachman, 1994; Meriggioli & Sanders, 2009). In most cases, it is caused by pathogenic autoantibodies directed towards the skeletal muscle acetylcholine receptor (AChR) (Patrick & Lindstrom, 1973) while in others, non-AChR components of the postsynaptic muscle endplate, such as the muscle-specific receptor tyrosine kinase (MUSK), might serve as targets for the autoimmune attack (Hoch et al., 2001). Although the precise origin of the autoimmune response in MG is not known, genetic predisposition and abnormalities of the thymus gland such as hyperplasia and neoplasia could have an important role in the onset of the disease (Berrih et al., 1984; Roxanis et al., 2001).

Several diseases affect muscles: they are classified as acquired (e.g. dermatomyositis and polymyositis) and hereditary (e.g. myotonic disorders and myopaties) forms. Among the myopaties, muscular dystrophies are characterized by the primary wasting of skeletal muscle, caused by mutations in the proteins that form the link between the cytoskeleton and the basal lamina (Cossu & Sampaolesi, 2007). Mutations in the dystrophin gene cause severe form of hereditary muscular diseases; the most common are Duchenne Muscular Dystrophy (DMD) and Becker Muscular Dystrophy (BMD). DMD patients suffer for complete lack of dystrophin that causes progressive degeneration, muscle wasting and death into the second/third decade of life. Beside, BMD patients show a very mild phenotype, often asymptomatic primarily due to the expression of shorter dystrophin mRNA transcripts that maintain the coding reading frame. DMD patients muscles show absence of dystrophin and presence of endomysial fibrosis, small fibers rounded and muscle fiber degeneration/regeneration. Untreated, boys with DMD become progressively weak during their childhood and stop ambulation at a mean age of 9 years, later with corticosteroid treatment (12/13 yrs). Proximal weakness affects symmetrically the lower (such as quadriceps and gluteus) before the upper extremities, with progression to the point of wheelchair dependence. Eventually distal lower and then upper limb weakness occurs. Weakness of neck flexors is often present at the beginning, and most patients with DMD have never been able to jump. Wrist and hand muscles are involved later, allowing the patients to keep their autonomy in transfers using a joystick to guide their wheelchair. Musculoskeletal contractures (ankle, knees and hips) and learning difficulties can complicate the clinical expression of the disease. Besides this weakness distribution in the same patient, a deep variability among patients does exist. They could express a mild phenotype, between Becker and Duchenne dystrophy, or a really severe form, with the loss of deambulation at 7-8 years. Confinement to a wheelchair is followed by the development of scoliosis, respiratory failure and cardiomyopathy. In 90% of people death is directly related to chronic respiratory insufficiency (Rideau et al., 1983). The identification and characterization of dystrophin gene led to the development of potential treatments for this disorder (Bertoni, 2008). Even if only corticosteroids were proven to be effective on DMD patient (Hyser and Mendell, 1988), different therapeutic approaches were attempted, as described in detail below (see section 7).

The identification and characterization of the genes whose mutations caused the most common neuromuscular diseases led to the development of potential treatments for those disorders. Gene therapy for neuromuscular disorders embraced several concepts, including replacing and repairing a defective gene or modifying or enhancing cellular performance, using gene that is not directly related to the underlying defect (Shavlakadze et al., 2004). As an example, the finding that DMD pathology was caused by mutations in the dystrophin gene allowed the rising of different therapeutic approaches including growth-modulating agents that increase muscle regeneration and delay muscle fibrosis (Tinsley et al., 1998), powerful antisense oligonucleotides with exon-skipping capacity (Mc Clorey et al., 2006), anti-inflammatory or second-messenger signal-modulating agents that affect immune responses (Biggar et al., 2006), agents designed to suppress stop codon mutations (Hamed, 2006). Viral and non-viral vectors were used to deliver the full-length - or restricted versions - of the dystrophin gene into stem cells; alternatively, specific antisense oligonucleotides were designed to mask the putative splicing sites of exons in the mutated region of the primary RNA transcript whose removal would re-establish a correct reading frame. In parallel, the biology of stem cells and their role in regeneration were the subject of intensive and extensive research in many laboratories around the world because of the promise of stem cells as therapeutic agents to regenerate tissues damaged by disease or injury (Fuchs and Segre, 2000; Weissman, 2000). This research constituted a significant part of the rapidly developing field of regenerative biology and medicine, and the combination of gene and cell therapy arose as one of the most suitable possibility to treat degenerative disorders. Several works were published in which stem cell were genetically modified by ex vivo introduction of corrective genes and then transplanted in donor dystrophic animal models.

Stem cells received much attention because of their potential use in cell-based therapies for human disease such as leukaemia (Owonikoko et al., 2007), Parkinsons disease (Singh et al., 2007), and neuromuscular disorders (Endo, 2007; Nowak and Davies, 2004). The main advantage of stem cells rather than the other cells of the body is that they can replenish their numbers for long periods through cell division and, they can produce a progeny that can differentiate into multiple cell lineages with specific functions (Bertoni, 2008). The candidate stem cell had to be easy to extract, maintaining the capacity of myogenic conversion when transplanted into the host muscle and also the survival and the subsequent migration from the site of injection to the compromise muscles of the body (Price et al., 2007). With the advent of more sensitive markers, stem cell populations suitable for clinical experiments were found to derive from multiple region of the body at various stage of development. Numerous studies showed that the regenerative capacity of stem cells resided in the environmental microniche and its regulation. This way, it could be important to better elucidate the molecular composition cytokines, growth factors, cell adhesion molecules and extracellular matrix molecules - and interactions of the different microniches that regulate stem cell development (Stocum, 2001).

Several groups published different works concerning adult stem cells such as muscle-derived stem cells (Qu-Petersen et al., 2002), mesoangioblasts (Cossu and Bianco, 2003), blood- (Gavina et al., 2006) and muscle (Benchaouir et al., 2007)-derived CD133+ stem cells. Although some of them are able to migrate through the vasculature (Benchaouir et al., 2007; Galvez et al., 2006; Gavina et al., 2006) and efforts were done to increase their migratory ability (Lafreniere et al., 2006; Torrente et al., 2003a), poor results were obtained.

Embryonic and adult stem cells differ significantly in regard to their differentiation potential and in vitro expansion capability. While adult stem cells constitute a reservoir for tissue regeneration throughout the adult life, they are tissue-specific and possess limited capacity to be expanded ex vivo. Embryonic Stem (ES) cells are derived from the inner cell mass of blastocyst embryos and, by definition, are capable of unlimited in vitro self-renewal and have the ability to differentiate into any cell type of the body (Darabi et al., 2008b). ES cells, together with recently identified iPS cells, are now broadly and extensively studied for their applications in clinical studies.

Embryonic stem cells are pluripotent cells derived from the early embryo that are characterized by the ability to proliferate over prolonged periods of culture remaining undifferentiated and maintaining a stable karyotype (Amit and Itskovitz-Eldor, 2002; Carpenter et al., 2003; Hoffman and Carpenter, 2005). They are capable of differentiating into cells present in all 3 embryonic germ layers, namely ectoderm, mesoderm, and endoderm, and are characterized by self-renewal, immortality, and pluripotency (Strulovici et al., 2007).

hESCs are derived by microsurgical removal of cells from the inner cell mass of a blastocyst stage embryo (Fig. 1). The ES cells can be also obtained from single blastomeres. This technique creates ES cells from a single blastomere directly removed from the embryo bypassing the ethical issue of embryo destruction (Klimanskaya et al., 2006). Although maintaining the viability of the embryo, it has to be determined whether embryonic stem cell lines derived from a single blastomere that does not compromise the embryo can be considered for clinical studies. Cell Nuclear Transfer (SCNT): Nuclear transfer, also referred to as nuclear cloning, denotes the introduction of a nucleus from an adult donor cell into an enucleated oocyte to generate a cloned embryo (Wilmut et al., 2002).

ESCs differentiation. Differentiation potentiality of human embryonic stem cell lines. Human embryonic stem cell pluripotency is evaluated by the ability of the cells to differentiate into different cell types.

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Stem Cell Therapy for Neuromuscular Diseases | InTechOpen

Center of Regenerative Orthopedics in South Florida Now Offering Stem Cell Therapy to Help Avoid Hip and Knee …

Pompano Beach, Florida (PRWEB) March 30, 2015

The top stem cell therapy practice in South Florida, Center of Regenerative Orthopedics, is now offering procedures to help patients avoid the need for hip and knee replacement. The procedures are partially covered by insurance and are offered by a highly skilled, Board Certified Orthopedic doctor in an outpatient setting. Call (954) 399-6945 for more information and scheduling.

Stem cell procedures for joint arthritis and pain are now mainstream and represent a cutting edge option for patients. Most nonoperative joint treatments do not actually alter the course of the disease, rather, simply act as a proverbial bandaid for relief. Stem cells, on the other hand, have the capacity to actually repair and regenerate damaged tissue such as cartilage, tendon and ligament.

Degenerative and rheumatoid arthritis affects tens of millions of Americans. Stem cell procedures have been showing excellent results for pain relief and functional improvements in small studies. By having the procedures partially covered by insurance, it makes them convenient for the general public to obtain the cutting edge option.

Joint replacement should be considered a last resort option for treatment. While typically successful, there are potential complications and they are not meant to last forever. In addition, there is minimal downtime after the stem cell procedures. Joint replacements take months to recover from afterwards.

Center of Regenerative Orthopedics is located in Pompano Beach, and sees patients throughout South Florida as well as from all over the United States. Call (954) 399-6945 to schedule with the top stem cell clinic in South Florida.

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Center of Regenerative Orthopedics in South Florida Now Offering Stem Cell Therapy to Help Avoid Hip and Knee ...