Some stem cell methods closer to 'gold standard' than others
Posted: July 3, 2014 at 1:47 am
PUBLIC RELEASE DATE:
2-Jul-2014
Contact: Kristina Grifantini press@salk.edu Salk Institute
LA JOLLA-Researchers around the world have turned to stem cells, which have the potential to develop into any cell type in the body, for potential regenerative and disease therapeutics.
Now, for the first time, researchers at the Salk Institute, with collaborators from Oregon Health & Science University and the University of California, San Diego, have shown that stem cells created using two different methods are far from identical. The finding could lead to improved avenues for developing stem cell therapies as well as a better understanding of the basic biology of stem cells.
The researchers discovered that stem cells created by moving genetic material from a skin cell into an empty egg cell-rather than coaxing adult cells back to their embryonic state by artificially turning on a small number of genes-more closely resemble human embryonic stem cells, which are considered the gold standard in the field.
"These cells created using eggs' cytoplasm have fewer reprogramming issues, fewer alterations in gene expression levels and are closer to real embryonic stem cells," says co-senior author Joseph R. Ecker, professor and director of Salk's Genomic Analysis Laboratory and co-director of the Center of Excellence for Stem Cell Genomics. The results of the study were published today in Nature.
Human embryonic stem cells (hESCs) are directly pulled from unused embryos discarded from in-vitro fertilization, but ethical and logistical quandaries have restricted their access. In the United States, federal funds have limited the use of hESCs so researchers have turned to other methods to create stem cells. Most commonly, scientists create induced pluripotent stem (iPS) cells by starting with adult cells (often from the skin) and adding a mixture of genes that, when expressed, regress the cells to a pluripotent stem-cell state. Researchers can then coax the new stem cells to develop into cells that resemble those in the brain or in the heart, giving scientists a valuable model for studying human disease in the lab.
Over the past year, a team at OHSU built upon a technique called somatic cell nuclear transfer (the same that is used for cloning an organism, such as Dolly the sheep) to transplant the DNA-containing nucleus of a skin cell into an empty human egg, which then naturally matures into a group of stem cells.
Ecker, holder of the Salk International Council Chair in Genetics, teamed up with Shoukhrat Mitalipov, developer of the new technique and director of the Center for Embryonic Cell and Gene Therapy at OHSU, and UCSD assistant professor Louise Laurent to carry out the first direct comparison of the two approaches. The scientists created four lines of nuclear transfer stem cells all using eggs from a single donor, along with seven lines of iPS cells and two lines of the gold standard hESCs. All cell lines were shown to be able to develop into multiple cell types and had nearly identical DNA content contained within them.
Read this article:
Some stem cell methods closer to 'gold standard' than others
- Ying Liu discusses IPS cell therapy for ALS [Last Updated On: August 6th, 2011] [Originally Added On: August 6th, 2011]
- IPs cells Part 1 [Last Updated On: September 5th, 2011] [Originally Added On: September 5th, 2011]
- Stem Cell Implications for ALS (Amyotrophic Lateral Sclerosis) [Last Updated On: September 5th, 2011] [Originally Added On: September 5th, 2011]
- A Century of Stem Cells - Johns Hopkins Medicine [Last Updated On: September 5th, 2011] [Originally Added On: September 5th, 2011]
- Myelin Repair Foundation on Stem Cell Research [Last Updated On: September 6th, 2011] [Originally Added On: September 6th, 2011]
- Jeanne Loring talks about stem cells, part 2 [Last Updated On: September 6th, 2011] [Originally Added On: September 6th, 2011]
- IPs Cells Part 4 [Last Updated On: September 6th, 2011] [Originally Added On: September 6th, 2011]
- Embryonic Stem Cells From Skin: Making Old Cells Young [Last Updated On: September 7th, 2011] [Originally Added On: September 7th, 2011]
- National Medical Report [Last Updated On: September 7th, 2011] [Originally Added On: September 7th, 2011]
- National Medical Report [Last Updated On: September 7th, 2011] [Originally Added On: September 7th, 2011]
- Jeanne Loring talks about stem cells, part 3 [Last Updated On: September 7th, 2011] [Originally Added On: September 7th, 2011]
- Kristopher Nazor 2 [Last Updated On: September 9th, 2011] [Originally Added On: September 9th, 2011]
- Myelin Repair Foundation on Stem Cell Research [Last Updated On: September 9th, 2011] [Originally Added On: September 9th, 2011]
- Dr. Oz to Oprah and Michael J Fox: "The stem cell debate is dead." [Last Updated On: September 10th, 2011] [Originally Added On: September 10th, 2011]
- Dr. Oz to Oprah and Michael J Fox: "The stem cell debate is dead." [Last Updated On: September 10th, 2011] [Originally Added On: September 10th, 2011]
- Andalusian Stem Cell Bank [Last Updated On: September 10th, 2011] [Originally Added On: September 10th, 2011]
- IPs cells Part3 [Last Updated On: September 10th, 2011] [Originally Added On: September 10th, 2011]
- Andalusian Stem Cell Bank [Last Updated On: September 11th, 2011] [Originally Added On: September 11th, 2011]
- IPs Cells Part 4 [Last Updated On: September 11th, 2011] [Originally Added On: September 11th, 2011]
- iPS Stem Cell-Based Treatment of Epidermolysis Bullosa [Last Updated On: September 12th, 2011] [Originally Added On: September 12th, 2011]
- Jeanne Loring talks about stem cells, part 1 [Last Updated On: September 12th, 2011] [Originally Added On: September 12th, 2011]
- Jeanne Loring talks about stem cells, part 2 [Last Updated On: September 12th, 2011] [Originally Added On: September 12th, 2011]
- Jeanne Loring talks about stem cells, part 3 [Last Updated On: September 12th, 2011] [Originally Added On: September 12th, 2011]
- Kristopher Nazor 2 [Last Updated On: September 12th, 2011] [Originally Added On: September 12th, 2011]
- Stem Cell Implications for ALS (Amyotrophic Lateral Sclerosis) [Last Updated On: September 12th, 2011] [Originally Added On: September 12th, 2011]
- IPs cells Part 2 [Last Updated On: September 13th, 2011] [Originally Added On: September 13th, 2011]
- Cellular Reprogramming Stem Cell Domain Name For Sale! - CellularReprogramming.com [Last Updated On: September 14th, 2011] [Originally Added On: September 14th, 2011]
- Cellular Reprogramming Stem Cell Domain Name For Sale! - CellularReprogramming.com [Last Updated On: September 19th, 2011] [Originally Added On: September 19th, 2011]
- IPs cells Part 2 [Last Updated On: September 23rd, 2011] [Originally Added On: September 23rd, 2011]
- Healthy Body = Functional Body. Hollywood Look Side Effect: Healthy Function [Last Updated On: September 25th, 2011] [Originally Added On: September 25th, 2011]
- Hollywood, Smith [Last Updated On: October 1st, 2011] [Originally Added On: October 1st, 2011]
- Manning, Owens Try Stem Cell Therapy [Last Updated On: October 4th, 2011] [Originally Added On: October 4th, 2011]
- Epidermolysis Bullosa: Corrected iPS Stem Cell-Based Therapy - Video [Last Updated On: October 13th, 2011] [Originally Added On: October 13th, 2011]
- Spinal Cord Injury - Embryonic Stem Cells - Dr. Keirstead - Video [Last Updated On: October 15th, 2011] [Originally Added On: October 15th, 2011]
- Introduction to Stem Cells - Video [Last Updated On: October 15th, 2011] [Originally Added On: October 15th, 2011]
- Parkinson's Disease: Progress and Promise in Stem Cell Research - Video [Last Updated On: October 16th, 2011] [Originally Added On: October 16th, 2011]
- Visions Episode 92: Stem Cells Discovery - Video [Last Updated On: October 16th, 2011] [Originally Added On: October 16th, 2011]
- James Bond Sam Botta Roger Moore I Married A Beautiful Woman - Video [Last Updated On: October 17th, 2011] [Originally Added On: October 17th, 2011]
- James Bond Sam Botta Roger Moore I Married A Beautiful Woman - Video [Last Updated On: October 18th, 2011] [Originally Added On: October 18th, 2011]
- induced pluripotent stem (iPS) cells: Part 2 - Video [Last Updated On: October 25th, 2011] [Originally Added On: October 25th, 2011]
- TEACHERS OWN CALPERS/GID Managed Apartments, Teachers EVICTING Paraplegic - Video [Last Updated On: November 5th, 2011] [Originally Added On: November 5th, 2011]
- TEACHERS OWN CALPERS/GID Managed Apartments, Teachers EVICTING Paraplegic - Video [Last Updated On: November 6th, 2011] [Originally Added On: November 6th, 2011]
- Stem Cells Could Repair Heart [11-14-2011] - Video [Last Updated On: November 21st, 2011] [Originally Added On: November 21st, 2011]
- Advantages Seroquel - Video [Last Updated On: December 6th, 2011] [Originally Added On: December 6th, 2011]
- induced pluripotent stem (iPS) cells: Part 2 - Video [Last Updated On: December 6th, 2011] [Originally Added On: December 6th, 2011]
- Advantages Seroquel - Video [Last Updated On: December 6th, 2011] [Originally Added On: December 6th, 2011]
- stem cell research - Video [Last Updated On: December 7th, 2011] [Originally Added On: December 7th, 2011]
- Ian Wilmut discusses stem cell and direct cellular transformation therapy - Video [Last Updated On: December 7th, 2011] [Originally Added On: December 7th, 2011]
- Lec 22 | MIT 16.885J Aircraft Systems Engineering, Fall 2005 - Video [Last Updated On: December 7th, 2011] [Originally Added On: December 7th, 2011]
- Paralyzed rat walks with own stem cells in 11 wks. - Video [Last Updated On: December 7th, 2011] [Originally Added On: December 7th, 2011]
- Lec 22 | MIT 16.885J Aircraft Systems Engineering, Fall 2005 - Video [Last Updated On: December 8th, 2011] [Originally Added On: December 8th, 2011]
- Jeff Bluestone: Immune rejection of stem cell transplants - Video [Last Updated On: December 8th, 2011] [Originally Added On: December 8th, 2011]
- Advances in Stem Cell Research: Shinya Yamanaka - Video [Last Updated On: December 10th, 2011] [Originally Added On: December 10th, 2011]
- 2011 Summit: Stem Cells, Reprogramming and Personalized Medicine, Rudolf Jaenisch, MD - Video [Last Updated On: December 14th, 2011] [Originally Added On: December 14th, 2011]
- Parkinson's Disease: Advancing Stem Cell Therapies - 2011 CIRM Grantee Meeting - Video [Last Updated On: January 19th, 2012] [Originally Added On: January 19th, 2012]
- Parkinson's Disease: Advancing Stem Cell Therapies - 2011 CIRM Grantee Meeting - Video [Last Updated On: January 20th, 2012] [Originally Added On: January 20th, 2012]
- Professor Alan Trounson - World focus on stem cell research - Video [Last Updated On: January 26th, 2012] [Originally Added On: January 26th, 2012]
- Professor Alan Trounson - World focus on stem cell research - Video [Last Updated On: January 26th, 2012] [Originally Added On: January 26th, 2012]
- Researchers turn skin cells into neural precusors, bypassing stem-cell stage [Last Updated On: January 31st, 2012] [Originally Added On: January 31st, 2012]
- Skin cells turned into neural precusors, bypassing stem-cell stage [Last Updated On: February 1st, 2012] [Originally Added On: February 1st, 2012]
- “Wide-ranging applications for pluripotent stem cells” [Last Updated On: February 2nd, 2012] [Originally Added On: February 2nd, 2012]
- New era of medicine in the offing, says scientist [Last Updated On: February 5th, 2012] [Originally Added On: February 5th, 2012]
- EC transfers Basti Commissioner to pacify agitating IPS officers [Last Updated On: February 7th, 2012] [Originally Added On: February 7th, 2012]
- Radiation treatment transforms breast cancer cells into cancer stem cells [Last Updated On: February 14th, 2012] [Originally Added On: February 14th, 2012]
- Radiation treatment generates cancer stem cells from less aggressive breast cancer cells, study suggests [Last Updated On: February 14th, 2012] [Originally Added On: February 14th, 2012]
- Radiation Treatment Generates Cancer Stem Cells from Less Aggressive Breast Cancer Cells [Last Updated On: February 14th, 2012] [Originally Added On: February 14th, 2012]
- Life Technologies Scientist Uma Lakshmipathy presents, "Solving Challenges in the Generation of Induced Pluripotent ... [Last Updated On: February 14th, 2012] [Originally Added On: February 14th, 2012]
- Radiation therapy transforms breast cancer cells into cancer stem cells [Last Updated On: February 14th, 2012] [Originally Added On: February 14th, 2012]
- Radiation treatment transforms breast cancer cells into cancer stem cells [Last Updated On: February 14th, 2012] [Originally Added On: February 14th, 2012]
- Radiation treatment generates cancer stem cells from less aggressive breast cancer cells, study suggests [Last Updated On: February 14th, 2012] [Originally Added On: February 14th, 2012]
- Life Technologies Scientist Uma Lakshmipathy presents, "Solving Challenges in the Generation of Induced Pluripotent ... [Last Updated On: February 15th, 2012] [Originally Added On: February 15th, 2012]
- Radiation Treatment Generates Cancer Stem Cells from Less Aggressive Breast Cancer Cells [Last Updated On: February 15th, 2012] [Originally Added On: February 15th, 2012]
- Horizon in new super-cell elite [Last Updated On: February 16th, 2012] [Originally Added On: February 16th, 2012]
- albuterol adverse effects - Video [Last Updated On: February 21st, 2012] [Originally Added On: February 21st, 2012]
- Presentations at the Society of Toxicology Annual Meeting Demonstrate Superior Predictivity of Cellular Dynamics ... [Last Updated On: March 8th, 2012] [Originally Added On: March 8th, 2012]
- A new approach to treating type I diabetes? Gut cells transformed into insulin factories [Last Updated On: March 11th, 2012] [Originally Added On: March 11th, 2012]
- New approach to treating type 1 diabetes? Transforming gut cells into insulin factories [Last Updated On: March 11th, 2012] [Originally Added On: March 11th, 2012]
- New approach to treating type 1 diabetes? Transforming gut cells into insulin factories [Last Updated On: March 11th, 2012] [Originally Added On: March 11th, 2012]
- A new approach to treating type I diabetes? Gut cells transformed into insulin factories [Last Updated On: March 11th, 2012] [Originally Added On: March 11th, 2012]
- Gut cells transformed into insulin factories 'could help to treat type I diabetes' [Last Updated On: March 12th, 2012] [Originally Added On: March 12th, 2012]