One of the most popular methods for the insertion of therapeutic genes into cells to treat the disease is to transport using a virus that has been stripped of their infectious properties. But non-infectious virus can still sometimes trigger immune responses dangerous.A team of Johns Hopkins Medicine proposes an alternative method for transporting large therapies cellsincluding genes and even gene-CRISPR system edition. It is a nano-container made of a polymer that biodegrades once their inside the cell, triggering therapy. The researchers describe the invention in the journal Advances Science.The team, led by biomedical engineer Jordan Green, Ph.D., was inspired by viruses, which have many properties that make them ideal vehicles. They have both negative and positive charges, for example, allowing the cells to approach them. Thus Green and his colleagues developed a polymer containing four molecules with positive and negative charges. I used to make a container that interacts with the cell membrane and eventually enveloped by it.How ICON, Lotus and Bioforum are improving the efficiency of modern studio with EDCCRO are often at the forefront of adopting new technologies to make clinical trials more efficient. Listen how ICON, Lotus Clinical Research, and Bioforum are accelerating the basis of building data and automating tasks management information data.RELATED: Could a grape-based compound to improve the efficiency of gene therapy?Hopkins researchers conducted four experiments to test the nanocontainers travel in cells and deliver complex therapies once inside. First, a small protein packaged in the polymeric material and mixed with mouse kidney cells in a laboratory dish. The use of fluorescent tags confirmed that the protein made in the cell. Experiment with immunoglobulinand noted a medicinehuman much larger than the 90% of renal cell received treatment was then repeated.From there, they made the payload, even bulkier packaging the nanocontainers with gene-CRISPR system edition. With the help of fluorescent signals that were able to confirm that CRISPR was to work once inside the cells, the inactivation of a gene 77% of the time.Thats pretty effective considering with other genes editing systems, it is possible to obtain the result of cutting right genes less than 10 percent of the time, said graduate student Rui Yuan said in a statement.Finally, the Hopkins researchers injected CRISPR components in mouse models of brain cancer using polymer nanocontainers. Again evidence that had taken place editing gene was successful.The development of improved methods for gene therapy is a priority in the field. In October, for example, scientists at Scripps Research describes a way to use a small molecule called caraphenol A to reduce levels of transmembrane induced by interferon (IFITM) proteins, which could, in turn, will allow viral vectors to pass more easily into cells. And earlier this year, an Italian equipment described a method for including the CD47 protein lentiviral vectors to improve the transfer of therapeutic genes into cells of the liver.The next step for researchers Hopkins Rui and green is to improve the stability of nanocontainers so they can be injected into the bloodstream. They hope to be able to direct them to cells that have certain genetic markers, it was reported.

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Hopkins invented equipment nonviral system for gene therapy in cells - Market Research Feed

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