Genetic changes tracked as bacteria become a fatal infection

Posted: March 6, 2012 at 7:42 pm

Eight mutations occurred in the common bacteria Staphylococcus aureus as it turned from an innocuous resident inside one persons nose into a fatal blood infection, an Oxford University study has found.

The study, which sequenced the complete DNA of the bacteria at regular time intervals, was able to identify for the first time the genetic changes that accompanied the transition to a dangerous infection.

Understanding the biological causes of serious bacterial infections could help guide screening in hospitals, and could inform efforts to develop vaccines against such infections. The study is published in the journal PNAS and was carried out in partnership with Oxford University Hospitals NHS Trust through the National Institute of Health Research (NIHR) Oxford Biomedical Research Centre.

"We have observed a significant genetic change in the bacteria corresponding with the development of a fatal blood infection," said Dr Bernadette Young of the Nuffield Department of Clinical Medicine at Oxford University, one of the lead researchers.

"It is one case study we simply dont know how much these results will be mirrored in others. But it could be a step towards identifying genetic changes that may be important in driving infection generally."

S. aureus is common, with one in four people carrying it inside the nose without any symptoms. Occasionally S. aureus can cause serious invasive infections, such as blood poisoning. Blood poisoning can develop as a complication of a skin infection, or via medical equipment that goes inside the body such as a feeding tube or catheter. But sometimes it appears to occur spontaneously, and it is thought that this can happen when nasal bacteria change in some way to allow invasive disease.

The Oxford researchers set out to understand more about the carriage of S. aureus inside the nose. They enrolled over 1,100 adults at GP practices in Oxfordshire and carried out nasal swabs. 360 people were found to carry S. aureus, who were then monitored with regular nasal swabs.

However, one elderly participant became ill a number of months into the study. The participant developed a serious condition requiring treatment, but soon after became unwell with blood poisoning and later passed away.

Complete DNA sequencing of the bacteria from the patients blood showed just a handful of changes from the bacteria in nose swabs before the patient became ill. It was the same bug causing infection that had been carried in the nose. But the few DNA changes may have been significant.

Eight new mutations were detected in the bacterial DNA from the patients blood. Four of the mutations cut genes short and are likely to have impaired the functions of bacterial proteins. One of these was in a protein that regulates stress response and virulence in S. aureus and could conceivably be connected to a biological change leading to infection.

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Genetic changes tracked as bacteria become a fatal infection

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