Gene-Silencing Technique Targets Scarring

A biotech company founded by Craig Mello, codiscoverer of RNAi, brings its first self-delivering interfering RNA to clinical trial.

A clinical trial of a gene-silencing treatment for reducing excessive scarring is now underway, bringing the number of active clinical trials for the as-yet-unproven gene-silencing process known as RNA interference to nearly 20.

Massachusetts-based RXi Pharmaceuticals has developed a chemically modified version of the small interfering RNAs that drive the biochemical process of RNA interference, or RNAi.

RNAi was discovered in 1998 and has since exploded as a research tool for turning off genes of choice in lab settings. Soon thereafter, biotech and pharmaceutical companies turned their attention to siRNAsshort pieces of RNA, DNA's cousin, that can prevent the activity of the specific gene they complementas a potential therapeutic tool, but so far no one has successfully commercialized the technology. The technology holds the potential to reduce the effects of almost any gene in a human cell, if only the interfering RNAs can get inside.

"Because RNA does not normally pass through the membrane of the cell, that delivery step is a major challenge," says Phil Sharp, a molecular biologist at MIT and a cofounder of an RNAi company called Alynylam (Sharp previously discussed the therapeutic potential of RNAi with Technology Review). Researchers must also strike a balance between the potency of the therapy in targeted cells and side-effects in other cells, he says.

A skin treatment reduces some of the delivery challenge because the target area is easily accessible. RXi injects its scar therapy directly under the outermost layers of the skin, delivering a large amount of the treatment to the target area, thereby reducing the risk of side effects to other parts of the body.

On a molecular level, the company modifies its interfering RNAs so that they are absorbed by the membrane of a cell. The company adds cholesterol molecules, which are normal components of cell membranes, to the RNAs so that the molecule will be more readily absorbed. Once in the membrane, the cholesterol breaks from the RNA molecule, which can then enter the cell body. RXi's version of siRNAs are also shorter than conventional siRNAs and have been chemically modified to resist the enzymatic wear and tear inside cells.

The scar-treating compound was designed to reduce the expression of a protein called connective tissue growth factor, or CTGF, which regulates several biological pathways involved in wound healing and scar formation. The protein drives production of collagen and other cellular support molecules referred to as matrix proteins.

In some people, CTGF overproduces these components, resulting in excessive scarring such as raised scars or larges scars called keloids. RXi's CEO Geert Cauwenbergh says the company's interfering RNA reduces scaring while still allowing CTGF to perform its wound-healing properties. "The interfering RNA doesn't silence [CTGF] 100 percentit continues to have some effect, but it's not excessive," he says.

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Gene-Silencing Technique Targets Scarring

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